Features:

• Source: 200 Hz frequency tripled Nd:YAG laser (355 nm) supports: o Matrix-assisted laser desorption/ionization (MALDI, positive and negative ion modes)o Laser desorption/ionization (LDI, positive ion mode)o 10 µm laser spot size for MALDI imaging (FlexImaging software installed)• Plates: 384-well stainless steel plate, Bruker AnchorChip, Hudson Surface technology plate (hydrophobic and untreated)• Mass analyzer: linear time-of-flight or reflectron time-of-flighto Mass Range: m/z 400-150,000 (linear, MALDI);o m/z 200-8,000 (reflectron, MALD/LDI)   • Mass accuracy: (all assume quadratic curve fit with 4 calibrants):o Reflectron, external calibration: ±20 ppm, ±5 ppm internal cal.o Linear, ext. cal. (m/z < 3000): ±50 ppm, ±20 ppm int. cal.o Linear, ext. cal. (m/z < 3000): ±150 ppm, ±100 ppm int. cal.• Resolving Power:o Reflectron: 12,000 @ m/z 2465 (20,000 possible for narrow ranges)o Linear: 4,000 @ m/z 2465; 1,000 @ m/z 12,361; 100 @ 66 kDa• Sensitivity: low fmol for a single peptide, low pmol for large polymers/proteins