Leica TCS SP8 Dive confocal/multiphoton microscope
Model: Leica SP8
Function: Leica TCS SP8 Dive confocal/multiphoton microscope has confocal capabilities equivalent to those of the system described above, but is mounted on an inverted stand. This system is also equipped with several features that extend our capabilities for intravital microscopy. First, it is equipped with two IR lasers, a Spectra-physics MaiTai DeepSee laser and a Spectra-physics Insight laser system that extends multiphoton excitation out to 1300 nm, improving intravital microscopy of red fluorescent proteins. The two-laser system also facilitates simultaneous excitation of multiple, spectrally separated probes. Second, this system is configured with 4 spectral detectors on the non-descanned pathway (2 PMTs and 2 HyD detectors), improving detection and distinction of multiple probes. Finally, this system is equipped with a two channel 2-photon ISS Fast FLIM fluorescence lifetime system and a motorized stage supporting tile acquisitions and time-lapse multiple filed studies.
Contact:
- Unit: IUSM Indianapolis
- Campus: School of Medicine
- Resource Type: Equipment
- Contact Name: Gosia Kamocka
- Contact Email: mkamocka@iu.edu
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