ISS Alba Fast FLIM microscope system
Model: ISS Alba Fast FLIM microscope system
Function: ISS Alba Fast FLIM system is a high-speed fluorescence imaging system that supports frequency-domain-based fluorescence lifetime imaging, fluorescence correlation microscopy and PIE. It is mounted on an Olympus IX71 microscope stand equipped with Olympus 10x/0.4, 20x/0.75, 40x/1.3 oil, and 60x/1.2W objective lenses coupled to a piezo stepper motor. The ISS FastFLIM system is coupled to the Alba LCSM. The lasers (10mW 405nm and 5mW 448nm diode lasers, and a 200 mW Supercontinuum White Light Laser) are modulated by the FastFLIM system at frequencies of 10 MHz or higher, and measurements can be analyzed using multiple digital harmonics. FRET FLIM measurements can be acquired for cyan, green or yellow donor probes, and the donor/acceptor signals are detected with two identical APDs. The APDs are capable of single-molecule detection, and the system is equipped with the ISS Alba FCS module. This enables one channel FCS or two channel cross-correlation FCS (FCCS). The ISS VistaVision firmware is also optimized for pulsed interleaved excitation (PIE) that enables crosstalk-free measurements of FCCS and measurements for FRET-FLIM from two independent probes simultaneously. The IS VistaVision software is used for phasor analysis of the FLIM measurements. The system is equipped with a stage-top environmental control system maintaining live specimen at 37º C and 5% CO2
Features: ISS Alba Fast FLIM system is a high-speed fluorescence imaging system that supports frequency-domain-based fluorescence lifetime imaging, fluorescence correlation microscopy and PIE. It is mounted on an Olympus IX71 microscope stand equipped with Olympus 10x/0.4, 20x/0.75, 40x/1.3 oil, and 60x/1.2W objective lenses coupled to a piezo stepper motor. The ISS FastFLIM system is coupled to the Alba LCSM. The lasers (10mW 405nm and 5mW 448nm diode lasers, and a 200 mW Supercontinuum White Light Laser) are modulated by the FastFLIM system at frequencies of 10 MHz or higher, and measurements can be analyzed using multiple digital harmonics. FRET FLIM measurements can be acquired for cyan, green or yellow donor probes, and the donor/acceptor signals are detected with two identical APDs. The APDs are capable of single-molecule detection, and the system is equipped with the ISS Alba FCS module. This enables one channel FCS or two channel cross-correlation FCS (FCCS). The ISS VistaVision firm
Contact:
- Unit: IUSM Indianapolis
- Campus: School of Medicine
- Resource Type: Equipment
- Contact Name: Connor Gulbronson
- Contact Email: cgulbron@iu.edu
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